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Flag-Tag Mouse mAb(2C5)

 

Cat#： TDY001   |   Source： Mouse   |   Type： Monoclonal   |
Applications: WB, IP, IF   Reactivity: N/A
MW.(KDa): N/A Isotype: IgG1

Applications:          WB-Western blot     IHC-Immunochemistry       IF-Immunofluorescence     IP-Immunoprecipitation     ChIP-Chormatin Immunoprecipitation

Reactivity:    H-Human R-Rat M-Mouse Mk-Monkey Dg-Dog Ch-Chicken Hm-Hamster Rb-Rabbit Sh-Sheep Pg-Pig
 
The DYKDDDDK peptide (Flag-tag) is a small component of an epitope which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. It has been used extensively as a general epitope tag in expression vectors. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.A Flag-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a Flag-tag to this protein allows one to follow the protein with an antibody against the Flag sequence. 
Applications Specifications Alternative Names

 

IP

 

IP antibody use：5ug Flag Mouse IgG1 per ml Lysate，WB 1:5000

1、untransfected 293 cell lysate

2、transfected 293 cell lysate with Flag-tag fusion protein

3、IP (transfected 293+ normal Mouse IgG+Protein G agarose)

4、IP (transfected 293+anti- Flag mAb+ Protein G agarose)

5、IP (transfected 293+Protein G)

6、Recombinant protein (E.coli)


Western Blot

 

1ug Flag fusion protein+ Primary antibody dilution at 1:10,000.

 

 

IF

 

IF analysis of 293 cells transfected with a Flag-tag protein, using TDYbio anti-FlagTag Mouse mAb at a 1:2000 dilution (blue DAPI, red anti-Flag)

 

 

Size:
TDY001C-50μL ￥800
TDY001F-100μL ￥1500
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